吸收物体的溶解和重新折叠由洗剂进行
Tsutomu Arakawa1, Teruo Akuta2, Daisuke Ejima3
1Alliance Protein Laboratories, 13380 Panter Road, San Diego, CA, 92130, USA.
Protein expression and purification
|August 9, 2025
概括
本综述侧重于从纳入体中重新折叠复合蛋白,这是生物制药开发中的一个常见挑战. 详细介绍了使用洗剂,性pH值和压力的有效策略,以成功进行蛋白质重新折叠.
科学领域:
- 生物化学 生化学
- 生物技术是生物技术.
- 蛋白质工程是指蛋白质工程.
背景情况:
- 再组合蛋白对于生物试剂和生物制药至关重要.
- 在包含体中表达的蛋白质通常需要重新折叠以获得功能.
研究的目的:
- 审查从纳入体中重新组合蛋白质的重新折叠方法.
- 为了比较各种溶解和重新折叠技术.
主要方法:
- 专注于使用洗剂 (例如,二硫酸盐,sarkosyl) 的溶解和重新折叠.
- 讨论了诸如化学伴侣,修改的溶解缓冲器和融合标签等策略,以增强可溶性表达.
- 探索性pH值和压力辅助重新折叠技术.
主要成果:
- 基于洗剂的方法对于蛋白质重新折叠是有效且具有成本效益的.
- 在重新折叠过程中,缓慢或逐步清除除色剂/洗剂对于保持蛋白质溶解性至关重要.
- 性pH和压力重新折叠也受益于缓慢的调整和诸如瓜尼丁化之类的添加剂.
结论:
- 通过优化溶解和重新折叠协议,可以从包含体中成功地重新折叠复合蛋白.
- 基于洗剂的溶解和重新折叠提供了一个实用和经济的方法.
- 仔细控制工艺参数,如pH值和变质剂去除率,对于高产的功能性蛋白质至关重要.
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