在干旱压力下,开发和选择稳定表达的参考基因,以在Ribes odoratum中实现表达正常化
Jun Xu1, Ting Li1, Xiaowen Liu1,2
1Qinghai Academy of Agriculture and Forestry, Qinghai University, Xining, 810016, China.
Scientific reports
|August 9, 2025
概括
确定稳定的内部参考基因对于Ribes odoratum中的基因功能研究至关重要. 这项研究在干旱压力下验证了定量PCR (qPCR) 的参考基因,发现集群-37.22228和集群-37.22646是最可靠的.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 遗传学 是一个遗传学.
背景情况:
- 使用定量PCR (qPCR) 精确的基因表达分析依赖于稳定的内部参考基因进行正常化.
- 对Ribes odoratum中的参考基因进行的有限验证研究阻碍了可靠的基因功能分析,特别是在干旱等环境压力下.
研究的目的:
- 在干旱压力条件下识别和验证稳定的参考基因,以使Ribes odoratum中的qPCR数据正常化.
- 建立一个可靠的基础,为未来的分子生物学研究 Ribes odoratum.
主要方法:
- 对Ribes odoratum进行了干旱压力治疗.
- 进行了转录组分析,以确定候选参考基因.
- 用多种算法 (Genorm,NormFinder,BestKeeper,Delta Ct) 评估了17个候选参考基因的表达稳定性.
主要成果:
- 确定了在干旱压力下表达稳定的多个基因.
- 集群-37.22228,集群-37.22646和集群-37.22897显示出最高的表达稳定性.
- 确定了集群-37.22228和集群-37.22646作为干旱压力下Ribes odoratum的最佳参考基因.
结论:
- 这项研究提供了首个在干旱压力下在Ribes odoratum中用于qPCR的验证参考基因的综合清单.
- 鉴定出稳定基因的参考基因将提高对该物种基因表达研究的准确性和可靠性.
- 这些发现将促进未来的分子研究和Ribes odoratum的遗传研究.
相关概念视频
01:17Gene Regulation During Sporulation
78
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
78
01:27Constitutive and Regulated Gene Expression
124
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
124
02:04Regulation of Transpiration by Stomata
29.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
29.1K
02:51mRNA Stability and Gene Expression
5.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.7K
02:02Responses to Salt Stress
13.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.4K