在微生物的光动力学无活化过程中,酸性细菌
Juliana Kafka Bilha1, Priscila Romero Mazzini Pereira1, Laura Beatriz Dos Santos Costa1
1Universidade Federal da Integração Latino-Americana, Caixa Postal 2044, Foz Do Iguaçu, PR, CEP 85866-000, Brazil.
概括
一种新型的阴性细菌 (Bchl-Cat) 通过光动力学疗法 (PDT) 有效地禁用了阴性细菌和Candida albicans. 这种光敏感剂为抗生素提供了一个有希望的替代品,避免了耐药性的发展.
科学领域:
- 生物化学 生物化学
- 摄影化学的使用.
- 微生物学 微生物学
背景情况:
- 光动力学疗法 (PDT) 使用光敏化剂 (PS) 产生反应性氧物种,导致细胞死亡.
- PDT是对微生物感染的有效治疗方法,由于缺乏耐药性发展,为抗生素提供了替代品.
研究的目的:
- 为了合成和评估一种新的性细菌 (Bchl-Cat) 来自细菌的光失活功效.
- 为了比较Bchl-Cat与非基衍生物 (Bchl-M) 对各种细菌和真菌病原体的疗效.
主要方法:
- 细菌菌素是从Rhodopseudomonas faecalis中提取出来的,并经过修改以产生细菌甲酸甲基 (Bchl-M) 和Bchl-Cat.
- 使用Bchl-Cat和Bchl-M对黄金葡萄球菌,Pseudomonas aeruginosa,大肠杆菌,沙门氏菌和Candida albicans进行了光灭活研究.
主要成果:
- Bchl-Cat证明了所有测试的微生物的显著光无活化.
- 在特定度和光剂量下观察到有效的格拉姆阴性细菌无活化:P. aeruginosa (3日志),大肠杆菌 (4日志) 和S. typhimurium (5日志).
- 与Bchl-M相比,Bchl-Cat在灭活阴性细菌方面显示出更高的疗效.
结论:
- 新型阴性细菌 (Bchl-Cat) 是用于光动力学治疗的强有力的光敏感剂.
- 与其非酸性对应物相比,Bchl-Cat对抗阴性细菌的抗微生物活性优越.
- 这些发现凸显了Bchl-Cat作为微生物感染的替代治疗剂的潜力.
相关概念视频
01:27Chemical Agents for Microbial Control
200
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
200
01:28Biological Methods for Microbial Control
202
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
202
01:26Physical Methods for Controlling Microbial Growth: Radiation and Filtration
218
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
218
01:28Anoxygenic Phototrophic Bacteria
148
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
148
01:29Methods for Controlling Microbial Growth
486
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
486
01:23Hand hygiene
4.1K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Hand washing...
4.1K