产母的禁食诱导变过程中肠道微生物群的致病性变化及其对脏免疫功能的影响
Hao Zhang1, Chenxu Wang2, Yujie Gong2
1College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, PR China; Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou, PR China.
Poultry science
|August 10, 2025
概括
产母的禁食诱导的变会改变肠道微生物群,并激活脏免疫力,影响病原体水平和免疫反应. 重新养使这些变化正常化,突出显示PI3K/AKT通路.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 禁食诱导化 (FIM) 用于提高老年的蛋产量和质量.
- FIM可能会增加病原体感染的风险,并激活脏免疫反应.
- 了解FIM对肠肠免疫的影响对于家禽健康至关重要.
研究的目的:
- 研究FIM对产母肠道微生物群,代谢物和脏免疫调节的影响.
- 在FIM期间分析炎症因子,免疫细胞标记物和PI3K/AKT通路的变化.
- 评估FIM对致病性和有益细菌和代谢物的影响.
主要方法:
- 甲基因组测序和代谢学来分析肠道微生物群和代谢物.
- 血液测试 (血清Ig) 和ELISA检测免疫标志物.
- 实时定量PCR和ELISA用于脏炎症因素和免疫细胞标记物.
- 对PI3K/AKT通路基因表达的分析.
主要成果:
- FIM改变了肠道微生物群,增加了非碳水化合物利用的病原体,减少了碳水化合物利用的病原体.
- 有益的肠道代谢物减少,然后增加;有害的代谢物显示相反的趋势.
- 血清中乙酸性白细胞增加和IgA降低;脏免疫反应激活,炎症因子增加 (NF-κB,IFN-β,iNOS,IL-1β,IFN-α,TNF-α,TGF-β,IL-1,IL-8).
- 禁食期间PI3K/AKT通路显著激活;在重新养后免疫参数正常化.
结论:
- FIM显著影响肠道微生物群,并在脏中引起强烈的免疫反应,由PI3K/AKT途径介导.
- 这些变化在重新养时在很大程度上是可逆的,这表明了动态的免疫适应.
- 这些发现为增强家禽免疫力和减少化期间感染提供了洞察力.
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