通过大米指转录因子Bsr-d1的DNA识别的分子机制
Lifeng Ji1, Jie Jiang1, Yiling Qin1
1State Key Laboratory of Maize Bio-breeding, Joint International Research Laboratory of Crop Molecular Breeding, Ministry of Agriculture Key Laboratory for Crop Pest Monitoring and Green Control, College of Plant Protection, China Agricultural University, Beijing, China.
Biochemical and biophysical research communications
|August 10, 2025
概括
米转录因子Bsr-d1使用其ZF1域专门结合DNA来激活植物免疫基因. 这项研究揭示了这种相互作用的结构基础,有助于在米中设计抗病能力.
科学领域:
- 植物分子生物学 植物分子生物学
- 结构生物学是结构生物学.
- 植物病理学 植物病理学
背景情况:
- 米转录因子Bsr-d1对于植物对Magnaporthe oryzae.e.的免疫是至关重要的.
- 在防过程中,BSR-d1激活过氧化酶基因并抑制过氧化 (H2O2).
- Bsr-d1与其目标DNA结合的确切机制仍然未被描述.
研究的目的:
- 阐明Bsr-d1识别和与其目标DNA结合的结构机制.
- 了解特定域和残留物在BSR-d1DNA相互作用中的作用.
- 为工程增强大米抗病能力提供基础.
主要方法:
- 使用AlphaFold3进行结构预测,以建模BSR-d1域.
- 凝过和生物层干扰测量 (BLI) 试验以确定DNA结合特异性.
- 位点定向突变发生,以验证关键残留物和参与结合的DNA基因.
主要成果:
- Bsr-d1 具有两个Cys2-His2 指域 (ZF1 和 ZF2).
- 只有ZF1域在过氧化酶基因促进体中专门结合了GTACT cis元素.
- 在ZF1中,关键残留物 (Y85,Q86,K92,R96,K97) 对于DNA结合至关重要,这种结合经过突变发生的证实.
结论:
- 这项研究揭示了Bsr-d1.1.介导的植物免疫中指DNA识别的结构基础.
- 这些发现突出了ZF1域和特定残留物在BSR-d1功能中的关键作用.
- 这项工作为开发基于BSR-d1的针对广谱大米病耐药性的策略奠定了基础.
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