从美国人参中提取糖蛋白的高效方法是通过天然的深溶性溶剂提取糖蛋白
1School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250012, China; School of Biological Science and Technology, University of Jinan, Jinan, 250012, China.
International journal of biological macromolecules
|August 10, 2025
概括
这项研究开发了一种高效的方法来从美国人参 (GAG) 中提取糖蛋白,使用微波辅助胆:酸 (ChCl:MalA). 这种新的方法显著增加了GAG产量和缩短了提取时间,也显示出卓越的肝保护活性.
科学领域:
- 自然产品化学 自然产品化学
- 生物化学 生物化学
- 提取技术 提取技术
背景情况:
- 美国人参中的葡萄糖蛋白以前没有报道过.
- 开发从药用植物中提取生物活性化合物的高效提取方法至关重要.
研究的目的:
- 开发一种有效的方法来从美国人参 (GAG) 中提取糖蛋白.
- 为了评估基于胆化物 (ChCl) 的天然深溶解剂 (NADES) 和各种提取技术.
- 通过响应表面方法来优化提取过程.
主要方法:
- 对三种基于ChCl的NADES (胆化物:酸,胆化物:乳酸,胆化物:糖醇) 的评价.
- 微波辅助,超声波辅助,加热和辅助的提取技术的比较.
- 使用响应表面方法优化微波辅助的ChCl:MalA提取.
主要成果:
- 微波辅助胆化物:酸 (ChCl:MalA) 证明了GAG提取的最高效率.
- 优化条件产生了19.91%的GAG,与传统方法相比,产量增加了47%,加工时间减少了87%.
- 提取的GAG具有中等分子量,狭窄的分布,高葡萄糖含量,典型的糖蛋白结构和粗略的形态.
- 在体外研究表明,通过微波辅助的ChCl:MalA.A.提取的GAG具有优越的肝保护活性.
结论:
- 微波辅助的ChCl:MalA提取是一种高效和有效的方法,用于从美国人参中获得GAG.
- 优化的方法在产量和时间效率上提供了显著的改进.
- 提取的GAG具有有益的生物活性特性,特别是肝脏保护作用.
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