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编码棕基转移酶的Caenorhabditis elegans spe-21基因对于精子生成是必要的

Saai Suryanarayanan¹, Dawn S Chen², Yamei Zuo¹

¹Waksman Institute and Department of Genetics, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ, 08854, USA.

Developmental biology

|August 10, 2025

概括

棕基转移酶SPE-21对于C. elegans的精子发育至关重要. 它使膜性器官融合成为可能,这对于创造功能性,受精能力强的精子来说至关重要.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

精子激活将圆形精子转化为运动精子.
这一过程涉及膜性有机体 (MO) 与C. elegans的血膜的融合.
MO融合对于精子表面蛋白质的结合和受精能力至关重要.

研究的目的:

鉴定和描述参与C. elegans.精子激活的spe-21基因.
阐明SPE-21在精子成熟过程中的分子功能.

主要方法:

对spe-21 (dhhc-5) 的基因鉴定,克隆和表征.
在体内和体外对spe-21无突变表型的分析.
使用mNeonGreen标记的SPE-21.1进行局部化研究.
与棕化相关的蛋白质功能的生物化学分析.

主要成果:

spe-21无基因突变体表现出严重的次生育能力.
突变精子体无法激活,显示MO融合和伪足形成的缺陷.
SPE-21是一种具有DHHC-CRD基因的跨膜蛋白,局部化到MOs.
SPE-21作为棕甲基转移酶起作用.

结论:

SPE-21对于C. elegans的精子激活和能够受精的精子的发展至关重要.
对于MO融合和适当的精子表面形成,SPE-21的棕基转移酶活性是必需的.

关键词:

这里是C. elegans.DHHC的图案是 DHHC 的图案.器官生物学器官生物学棕甲基转移酶的作用精子激活是精子的激活.囊泡融合是什么意思囊泡融合

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