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Updated: Sep 12, 2025

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
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一种来自Ligusticum chuanxiong Hort.的细胞分离方法. 适合获得高质量的RNA用于Smart-seq

Ruoshi Li1,2, Mengmeng Wu1,2, Shunlu Chen1,2

  • 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Liucheng Street, 611137, Chengdu, Sichuan, China.

Plant methods
|August 10, 2025
PubMed
概括

我们开发了一种用于植物单细胞RNA提取的简单方法,比激光捕获微解剖提高了质量. 这种技术为Smart-seq分析产生高质量的RNA,揭示了细胞类型特定的转录组.

关键词:
在Ligusticum chuanxiong Hort中使用.细胞隔离 细胞隔离激光捕捉微解剖是指激光捕捉的微解剖.抛部分的抛部分.智能-seqq是什么意思

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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