Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.1K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.1K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

688
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
688

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

3D Bioprinting of High-Grade Serous Ovarian Cancer Cells: Workflow for Gelatin-Based Bioink Formulation with Hyaluronic Acid and Printability Assessment.

ACS applied bio materials·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026
Same author

Towards greener-by-design fine chemicals. Part 2: technological frontiers.

Chemical Society reviews·2025
Same author

pH-Thermo Dual-Responsive Polymeric Nanoparticles for Women's Health: Dual Action Against Cervical and Ovarian Cancer Cells.

ACS applied materials & interfaces·2025
Same author

Drug Delivery from Nanogel Formulations: Mathematical Modeling of Different Release Profiles and Conditions.

Molecular pharmaceutics·2025
Same author

Fluorinated Redox-Responsive Cross-Linked Poly(amidoamine) G2 as Smart Theranostic Dendrimers.

Biomacromolecules·2025

相关实验视频

Updated: Sep 11, 2025

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

11.4K

3D打印的微流体色谱柱用于快速下游处理的开发.

Vladimir Matining1, Mario Messina1, Benedetta Sechi1

  • 1Department of Chemistry, Materials, and Chemical Engineering "Giulio Natta", Politecnico Di Milano, Piazza Leonardo Da Vinci 32, Milan, Italy.

Biotechnology journal
|August 11, 2025
PubMed
概括

3D打印使新的微流体色谱列能够进行高效的生物分子分析. 这项研究开发了一个3D打印的柱子用于酶表征,减少样品和树脂的要求.

关键词:
通过3D打印打印3D打印.这就是HETP.吸附平衡是一种吸附平衡.染色体学 染色体学 染色体学高通量选的高通量选微流体学 在微流体学方面

更多相关视频

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.1K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

相关实验视频

Last Updated: Sep 11, 2025

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

11.4K
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
13:36

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

4.1K
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.3K

科学领域:

  • 分析化学 分析化学
  • 化学工程是化学工程的重要组成部分.
  • 生物技术是生物技术.

背景情况:

  • 微流体设备在样品消耗减少和更快的分析方面具有优势.
  • 3D打印为制造复杂的微流体结构提供了一个多功能平台.

研究的目的:

  • 开发和描述一个3D打印的微流体色谱柱.
  • 为了评估该列对蛋白质特征的性能,特别是lyszyme.
  • 在微柱中建立蛋白质运输的机械模型.

主要方法:

  • 使用3D打印制造54μL微流体柱.
  • 用离子交换树脂包装列.
  • 使用酸标志物和lyszyme进行表征.
  • 吸附异热和轴向分散的分析.

主要成果:

  • 3D打印的柱子表现出良好的染色学特性 (孔度 ε=0.72,不对称系数 0.8-1.8).
  • 酶和能力测量为88.14g/L树脂.
  • 在高NaCl度 (≥300毫米) 的情况下,观察到从兰木尔到抗兰木尔吸附的过渡.
  • 轴分散系数被确定为6.7 x 10−9 m2/s.

结论:

  • 3D打印是一种可行的技术,用于创建高性能微流体色谱列.
  • 开发的微柱使得像lyszyme这样的生物分子能够在最小的样本和树脂中进行全面的表征.
  • 这种方法简化了过程开发,并解决了有限的生物分子可用性的挑战.