高凝血状态:对凝块波形分析,血栓生成和凝块扫描电子显微镜的研究
Maria Filomena Ruberto1, Silvia Marongiu2, Terenzio Congiu3,4
1From the Laboratory Department, Madonna del Rimedio Hospital, Oristano, Oristano, Italy (Ruberto).
Archives of pathology & laboratory medicine
|August 11, 2025
概括
凝块波形分析 (CWA) 检测高凝血状态,类似于血栓生成试验. 这种方法揭示了凝块质感的差异,为凝血障碍提供了新的见解.
科学领域:
- 血液学 血液学 血液学
- 医学诊断 医学诊断 医学诊断
背景情况:
- 凝块波形分析 (CWA) 为标准测试提供了对凝块过程的详细见解,例如前血时间 (PT) 和激活的部分血栓形成时间 (aPTT).
- 凝血仪中的光学检测系统可以在没有额外的试剂成本的情况下捕获CWA数据.
研究的目的:
- 确定CWA是否能够在各种临床条件中识别出高凝血状态,反映出血栓生成 (TG) 试验的能力.
- 用扫描电子显微镜 (SEM) 调查体外血栓的纹理差异.
主要方法:
- 收集了来自191名患者的数据,包括PT INR,aPTT比率,CWA,D-二次数,纤维素,·威尔布兰德因子 (vWF:Ag,vWF:RiCo),TG测定和SEM血块成像.
- 使用 MATLAB 软件对 SEM 图像进行了纹理分析.
主要成果:
- 与对照组相比,患有COVID-19,全身性硬化症和高或低的帕多瓦预测分数 (PPS) 的患者表现出较高的CWA,纤维素,D-二元和TG水平.
- 在这些患者群体中,SEM在体外发现了更厚的血栓.
- 在COVID-19患者中,vWF:Ag和vWF:RiCo水平最高.
结论:
- 在患有凝血风险的患者中,CWA有效地检测出高凝血状态,与TG测试相似.
- 在SEM分析中,在体外发现了不同的凝块纹理,可能揭示了潜在的病理.
- 虽然CWA目前只是一个研究工具,但它可能会发展成为一种临床上有价值的测试,增强PT和aPTT的结果.
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