超声波快速处理方法对氨酸嵌入组织中的蛋白质,DNA和RNA的影响
Xiaohong Li1,2, Jiadi Luo3,4, Qingchun Liang5,6
1Department of Pathology, Second Xiangya Hospital, Central South University, Changsha 410011. li1989@csu.edu.cn.
概括
超声波快速处理方法显著减少了组织准备时间,而不会损害蛋白质,DNA或RNA完整性. 这种验证的技术支持标准和分子病理学分析.
科学领域:
- 病理学 病理学 病理学
- 生物技术是生物技术.
- 分子生物学分子生物学
背景情况:
- 传统的嵌组织加工是耗时的.
- 快速超声波处理对组织生物分子的影响在很大程度上仍未被描述.
研究的目的:
- 评估超声波快速处理对嵌组织中蛋白质,DNA和RNA的影响.
- 将超声波快速处理与传统的病理分析方法进行比较.
主要方法:
- 使用了HE染色,免疫组织化学,DNA片段分析,HER2的FISH,EGFR/ALK的NGS和RT-PCR.
- 在各种瘤样本上比较超声波快速处理与传统方法.
主要成果:
- 超声波快速处理显著减少了样品准备时间.
- 来自HE染色,IHC,DNA分析,HER2 FISH,EGFR/ALK NGS和RT-PCR的结果在方法之间是一致的.
结论:
- 超声波快速处理是加快样本准备的有效方法.
- 这种技术保护了DNA,RNA和蛋白质的完整性,用于下游分析.
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