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Updated: Sep 11, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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红光诱导的氨酸修饰适用于蛋白质标记

Tomasz Wdowik1, Egor Fedorov1, Tina-Thien Ho2,3

  • 1Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, Warsaw 01-224, Poland.

ACS organic & inorganic Au
|August 11, 2025
PubMed
概括

这项研究引入了使用红光和氨酸催化剂进行生物结合的新方法,使生物环境中有效的氨酸修饰成为可能. 该技术提供了一种温和的,以水为基础的方法,用于在蛋白质中选择性氨酸转化.

关键词:
生物结合生物结合半氨酸 (cysteine) 是一种氨酸.光催化作用的光催化作用氨酸是氨酸中的一种.蛋白质是一种蛋白质.红灯是红色的 红灯是红色的硫烯的反应反应

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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