将基于NUPACK的模拟纳入课堂和实验室教学中,用于本科生化学的核酸混合化
Jinglin Fu1,2, Anthony Monte Carlo1, Doris Zheng1
1Department of Chemistry, Rutgers UniversityCamden, Camden, New Jersey 08102, United States.
Journal of chemical education
|August 11, 2025
概括
本研究介绍了一个基于NUPACK的计算实验室,用于在生物化学中教授核酸. 这种可适应的平台增强了对DNA结构和杂交的理解,促进了远程学习.
科学领域:
- 生物化学教育教育 生物化学教育
- 计算生物学 计算生物学
- 分子生物学分子生物学
背景情况:
- 随着COVID-19的流行,人们不得不转向远程学习,这凸显了对可适应的教育平台的需求.
- 传统的"湿实验室"活动在偏远环境中带来了挑战,需要创新的虚拟替代方案.
- 可访问和灵活的学习环境对于科学教育中的各种学生需求至关重要.
研究的目的:
- 使用NUPACK生物化学网络应用程序开发和描述关于核酸的教育模块.
- 创建一个虚拟的"干实验室"体验,补充讲座,并增强对核酸性质的理解.
- 为生物化学和分子生物学的教育工作者和学生提供灵活和可访问的学习工具.
主要方法:
- 利用NUPACK,一个免费的基于云的Web应用程序,来设计用于核酸教育的计算模块.
- 开发了包括融化温度,杂交平衡,自由能量和二次结构等主题的模块.
- 为生物化学讲座和实验室实施了计算机辅助的DNA实验,包括向高中生推广.
主要成果:
- 成功创建了基于NUPACK的教育模块,用于教导基本的核酸概念.
- 计算实验室提供了实践式的学习体验,提高了学生对核酸结构和杂交的理解.
- 在疫情期间成功过渡到远程学习,保持教育连续性.
- 扩展了计算DNA实验以吸引当地高中生,扩大了可访问性.
结论:
- 基于NUPACK的计算实验室提供了一种有效和可适应的方法,用于在生物化学中教授核酸.
- 虚拟"干实验室"练习提高了学生的理解,并为远程和面对面的学习提供了灵活性.
- 这种方法支持创新的在线教学方法,并为各种学习者扩大了对分子生物学教育的访问.
相关概念视频
Southern Blot
20.2K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
20.2K
DNA Packaging
104.4K
Overview
104.4K
In-situ Hybridization
9.6K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.6K
Nucleic acids
168.8K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
168.8K


