评估清除剂,固定和过程持续时间对心血管组织成像的影响,使用第二波生成和多光子模式
Maedeh Makki1, Zachary A Molander1, Sergio A Pineda-Castillo2
1Biomechanics and Biomaterials Design Laboratory (BBDL), Department of Bioengineering, The University of California, Riverside (UCR), Riverside, CA, United States.
Frontiers in bioengineering and biotechnology
|August 11, 2025
概括
醇基酸 (BABB) 是用于心血管组织成像的优质清除剂,增强透明度和信号深度. 在无标签的多光子显微镜中,形式素固定需要方法特定的优化,以获得最佳的结果.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 光学清除技术的使用
- 心血管组织分析分析
背景情况:
- 已建立的组织清除协议主要针对中枢神经系统.
- 对心血管组织进行有效的光学清除存在重大挑战.
- 无标签的多光子成像需要优化组织清除,以便深度可视化.
研究的目的:
- 评估猪左前下降动脉 (LADA) 的不同组织清除方法.
- 为了研究甲素固定和储存时间对组织清除的影响.
- 确定心血管组织无标签多光子和第二子生成成像的最佳条件.
主要方法:
- 醇甲酸 (BABB) 和糖清除方法的比较.
- 对不同形式素固定时间 (0-240分钟) 的评估.
- 在BABB中延长组织存储的评估 (长达14天).
- 对图像深度的组织保存和透明度的分析.
主要成果:
- 与糖醇相比,BABB显著提高了组织透明度和信号强度 (AF-AUC和SHG-AUC).
- 在储存14天后,BABB保持了组织完整性和光信号.
- 形式素固定改善了用糖醇保存的质量,但用BABB降低了透明度.
结论:
- BABB是用于心血管组织深度3D多光子/SHG成像的优质无标签清除剂.
- 根据所选择的清除方法调整固定参数对于最佳成像结果至关重要.
- 这项研究为推进心血管组织成像技术提供了必要的见解.
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