盐度压力诱导植物的相分离 BARENTSZ 形成凝结物
Peigang Li1,2,3, Lanfeng Wu1,2,3, Wenchao He1,3
1State Key Laboratory of Plant Diversity and Specialty Crops/State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
概括
植物BARENTSZ (BTZ) 蛋白在盐应激下形成凝结物,增强大米的盐度耐受性. 这种在进化过程中保存的相分离机制揭示了BTZ在植物非生物应激适应中的新角色.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 众所周知,巴伦茨 (BTZ) 蛋白质是外连结复合体 (EJC) 的关键组成部分,在动物中经历相分离 (PS).
- 植物中BTZ蛋白的作用和机制,特别是在非生物压力下,仍然在很大程度上未被探索.
研究的目的:
- 为了研究Oryza sativa BTZ (OsBTZ) 基因在米盐度耐受性中的功能.
- 为了阐明在盐应力下OsBTZ蛋白相分离的机制.
主要方法:
- 基因淘汰创建功能丧失的OsBTZ突变.
- 使用显微镜观察OsBTZ蛋白相分离和凝结物形成.
- 分析类域 (PrLD) 在OsBTZ阶段分离中的作用.
主要成果:
- 失去OsBTZ功能显著降低了大米对度压力的耐受性.
- OsBTZ蛋白质独立于其他EJC核心成员进行相分离,在盐应力下形成细胞质凝聚物.
- 进化保守的类域 (PrLD) 对于由NaCl引起的OsBTZ相分离至关重要.
结论:
- OsBTZ蛋白质在米盐度耐受性中起着至关重要的作用.
- OsBTZ相位分离形成应力颗粒,这些颗粒招募与盐耐受性相关的蛋白质.
- 这项研究突出了植物BTZ中PS的进化保守机制,用于非生物应激适应.
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