相关实验视频

Updated: Sep 11, 2025

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Published on: April 18, 2017

索克斯11基因会影响斑马鱼发育中的肠道神经系统中的神经元分化

Yuanyun Huang¹, Can Li¹, Ayyappa Raja Desingu Rajan¹

¹Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.

Proceedings of the National Academy of Sciences of the United States of America

|August 11, 2025

概括

Sox11a和Sox11b对于在斑马鱼发育中的肠道神经系统 (ENS) 中指定神经元亚型至关重要. 它们的耗尽通过影响增强器活性来减少抑制性运动神经元数量.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

脊椎动物的肠道神经系统 (ENS) 源自阴道神经细胞.
这些细胞迁移以填充整个肠道,分化为各种神经元亚型.

研究的目的:

研究转录因子sox11a和sox11b在斑马鱼内脏神经系统发育中的作用.
了解 sox11 对应物如何影响神经元亚型规范.

主要方法:

为了研究功能丧失效应,CRISPR-Cas9基因耗尽.
ATAC-seq和转录因子足迹用于识别Sox11结合位点.
记者基因测定与突变分析,以确认增强器功能.

主要成果:

在过渡神经细胞中,Sox11a和Sox11b的表达很高.
丢失sox11对应物会减少表达adcyap1b的抑制性运动神经元的数量.
在adcyap1b增强剂中确定了Sox11结合部位,它们对其活性至关重要.

结论:

在斑马鱼发育中的ENS中,Sox11a和Sox11b在指定抑制性运动神经元亚型方面发挥着至关重要的,以前未被认可的作用.
这些转录因子通过增强元件来调节神经元的分化,例如adcyap1b.

关键词:

细胞系细胞系的细胞系不同化的差异化差异化.肠道神经系统肠道神经系统神经峰的神经峰神经元神经元是一个神经元.

更多相关视频

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Published on: April 27, 2018

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Published on: February 28, 2014

相关概念视频

Pleiotropy

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...