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转录组广泛协会揭示了控制大豆种子重量的LncRNAs

Xiang Wang¹, Xinyu Jiang¹, Xiaobo Yuan¹

¹State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu, 210095, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|August 11, 2025

概括

长非编码RNAs (lncRNAs) 是植物发育的关键. 这项研究确定了调节大豆种子重量的lncRNAs,为提高作物产量提供了见解.

科学领域:

植物分子生物学植物分子生物学
遗传学是一个遗传学.
农业科学农业科学

背景情况:

长非编码RNAs (lncRNAs) 越来越多地被认为是植物生物过程中的关键调节者.
lncRNAs在调节种子重量的特定参与,这是作物产量的关键因素,仍然在很大程度上未经调查.

研究的目的:

系统地识别和描述与大豆种子重量变化相关的长非编码RNA (lncRNAs).
阐明 lncRNAs 在大豆种子发育和重量确定中的调节机制.

主要方法:

对238个大豆加入进行了全转录组关联研究 (TWAS),以确定有意义的lncRNAs.
对候选 lncRNAs 进行了功能验证,以评估它们在种子重量调节中的作用.
采用eQTL分析,了解种子发育过程中lncRNA表达的遗传控制.

主要成果:

分别在开花后14天和21天确定了175个和50个TWAS显著的lncRNAs.
通过调节关键种子发育基因,两种候选lncRNA被验证为种子重量的积极调节者.
发现了与种子重量相关的显著的lncRNA-lncRNA联合表达模块.
eQTL分析揭示了动态和静态eQTLs影响lncRNA表达,远端eQTLs显著影响表型变异.

结论:

长非编码RNA在通过转录调节发育途径来调节大豆种子重量方面发挥着重要作用.
邻近和远端eQTL的整合改善了种子重量的预测,突出了远端遗传效应的重要性.
这些发现为种子重量调节提供了新的分子见解,为提高作物产量的策略铺平了道路.

关键词:

在TWAS中,它是TWAS.我们的eQTL是eQTL.在 lncRNAs 中.种子重量种子重量豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆

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