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相关概念视频

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

496
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
496
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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相关实验视频

Updated: Sep 11, 2025

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
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计算领域分辨率连贯化学成像成像.

Shupeng Zhao1, Lea Chibani2, Edward Chandler3

  • 1Laboratoire Kastler Brossel, ENS-Université PSL, CNRS, Sorbonne Université, Collège de France, 24 rue Lhomond, Paris, France. spzhao0724@gmail.com.

Nature communications
|August 11, 2025
PubMed
概括

这项研究引入了一种新的连贯抗斯托克斯拉曼散射 (CARS) 方法来恢复丢失的光谱相信息. 这一突破使得用于先进诊断的无人工物,高速定量化学成像成为可能.

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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Direct Imaging of Laser-driven Ultrafast Molecular Rotation
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Direct Imaging of Laser-driven Ultrafast Molecular Rotation

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相关实验视频

Last Updated: Sep 11, 2025

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
08:51

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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科学领域:

  • 频谱学是一种光谱学.
  • 化学成像技术 化学成像技术
  • 生物医学诊断 生物医学诊断

背景情况:

  • 连贯的反斯托克斯拉曼散射 (CARS) 对于化学成像至关重要,但由于失去了光谱相,它在定量分析方面遇到了困难.
  • 获取光谱相位信息是提高CARS速度和准确性的关键.

研究的目的:

  • 开发一种新的CARS技术,用于在没有外部引用的情况下检索振动光谱相.
  • 为了实现没有文物,高速的定量化学成像.

主要方法:

  • 开发了一个无基准的干扰度宽带/探头CARS系统.
  • 从空间成像到频域光谱的自适应的计算相位检索.
  • 证明了受监督的压缩CARS显微镜.

主要成果:

  • 通过使用新的CARS方法成功获取振动光谱相位信息.
  • 实现了没有文物,高速的定量化学成像.
  • 验证了计算阶段检索的可转移性到光谱学.

结论:

  • 开发的CARS技术克服了定量化学成像方面的局限性.
  • 这一进步为各种科学领域的更快,更精确的化学分析铺平了道路.