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精细映射影响绵羊出生体重的遗传变异:使用低覆盖全基因组测序对3007个个体的GWAS

Ran Li¹, Yuheng Bai^1,2, Maqiang Zhao¹

¹Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Journal of animal science and biotechnology

|August 12, 2025

概括

低覆盖全基因组测序 (lcWGS) 确定了影响绵羊出生体重的新型遗传因素. 9号染色体上的一个关键的SNP显著增加了出生体重,为家畜的选择性繁殖提供了潜力.

科学领域:

动物遗传学动物遗传学
基因组学就是基因组学.
畜牧业生产畜牧业生产

背景情况:

出生的体重是牲畜中关键的经济特征,但其遗传基础尚未完全理解.
之前的研究受限于小样本大小和低密度的基因型阵列.

研究的目的:

用低覆盖全基因组测序 (lcWGS) 调查胡羊出生体重的遗传结构.
识别与出生体重相关的新型遗传变异和定量特征位点 (QTLs).

主要方法:

使用 lcWGS 进行基因型 3,007 个胡羊.
进行了全基因组关联研究 (GWAS) 和表达量化特征位置 (eQTL) 映射.
利用基于哈普洛型的精细映射来识别候选因果变异.

主要成果:

在染色体6和9上确定了两个新型的QTLs用于出生体重.
与PLAG1表达相关的9号染色体上的QTL显示出与异合体载体出生体重增加的显著关联 (增加9.85%).
染色体6上的二次QTL与FecB突变结合在一起,与降低出生体重有关,表明有类效应.

结论:

lcWGS是一种具有成本效益和高分辨率的方法,用于剖析牲畜中的复杂特征.
鉴定出的基因变异为羊的出生体重遗传学提供了洞察力.
这些发现支持在畜牧业生产中加速基因改进计划.

关键词:

出生的体重出生时的体重.在GWAS中,GWAS就是GWAS.在LcWGS中使用.在 QTL 时段,你会得到 QTL.绵羊是一种羊.

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