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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Sep 11, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
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超高通量单像素复合场显微镜与频谱声光学连贯编码 (FACE).

Daixuan Wu1, Yuecheng Shen2, Zhongzheng Zhu1

  • 1Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, Guangdong, 510006, China.

Light, science & applications
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概括

我们开发了一种新的单像素复杂场显微镜 (SPCM) 系统,可以显著提高可见光谱之外的成像速度和分辨率. 这种先进的光学成像技术克服了对实时,高通量应用的先前限制.

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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相关实验视频

Last Updated: Sep 11, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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科学领域:

  • 光学和光子学 在光学和光子学.
  • 显微镜的使用方法
  • 非可见光谱成像技术 不可见光谱成像

背景情况:

  • 单像素成像 (SPI) 提供了超出可见光谱的成像潜力,但由于模式投射和重建速度缓慢,其吞吐量较低.
  • 传统的SPI对于高速,高分辨率的成像任务是不够的.
  • 现有的系统在与非可见领域的实时复杂场监控作斗争.

研究的目的:

  • 开发一个超高通量单像素复杂场显微镜 (SPCM) 系统.
  • 为了实现实时复杂场域监控,超出可见光谱.
  • 为了克服传统SPI的吞吐量限制.

主要方法:

  • 使用频率声光学连贯编码 (FACE) 进行SPCM.
  • 在1030nm波长下运行系统.
  • 实现了高空间带宽-时间产品 (SBP-T) 以提高性能.

主要成果:

  • 展示了1.3×107的创纪录的SBP-T,显著超过之前的SPCM,SPI和近红外摄像机.
  • 实现了1000Hz的实时流媒体,80×81像素和3.76μm横向分辨率.
  • 成功拍摄了动态透明场景,活微生物,化学反应,以及通过散射介质.

结论:

  • 开发的基于FACE的SPCM系统为超出可见光谱的高速,高分辨率的复杂场成像提供了卓越的解决方案.
  • 这一进步显著提高了SPI性能,用于各种应用.
  • 该系统能够实时监控和成像在具有挑战性的非可见光谱范围.