大多数自细胞死亡研究缺乏因果关系的证据
Ali Burak Özkaya1, Yasmin Ghaseminejad1
1Faculty of Medicine, İzmir University of Economics, Turkey.
FEBS open bio
|August 12, 2025
概括
严格评估声称自细胞死亡 (ACD) 的研究发现了重大问题. 大多数研究都缺乏足够的证据,突出了对自研究中标准化方法和精确术语的需求.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 自对于细胞平衡至关重要,参与各种生理和病理过程.
- 虽然经常与细胞存活有关,但自细胞在细胞死亡 (自细胞死亡,ACD) 中的作用仍在争论中.
- 鉴定ACD是具有挑战性的,因为不一致的方法和术语.
研究的目的:
- 系统地评估2022年发表的声称证明自细胞死亡 (ACD) 的研究文章.
- 评估实验设计的严谨性和ACD所提供的证据的有效性.
- 确定常见的陷,并提出用于分类自和细胞死亡相互作用的标准化方法.
主要方法:
- 从2022年开始对104篇声称使用ACD的研究文章进行系统审查.
- 根据既定的标准进行评估:自的证据,细胞死亡,排除细胞亡和因果关系.
- 分析了自流量,细胞死亡的证据和自抑制的有效性.
主要成果:
- 只有12.5%的研究符合ACD的所有标准.
- 37.5%的人只提供了相关证据,而不是因果关系.
- 重要问题包括未能证明自流 (54.81%),依赖生命力丧失 (32.7%) 和无效的自抑制 (45.0%).
- 不一致的术语和错误分类的自介导细胞死亡是普遍存在的.
结论:
- 目前用于证明ACD的研究实践缺乏严格性,通常依赖于相关性而不是因果关系.
- 广泛的术语滥用和方法不一致性阻碍了ACD的可靠识别.
- 开发系统的工作流程和标准化方法对于进一步了解自在细胞死亡中的作用至关重要.
相关概念视频
01:18Autophagic Cell Death
3.6K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.6K
01:30Overview of Cell Death
7.7K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.7K
01:27Autophagy
4.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.6K
01:16Necrosis
4.8K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.8K
01:36Delivery Pathways to the Lysosome
7.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.2K
01:17Phagocytosis of Apoptotic Cells
4.0K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
4.0K