延迟血离心对血清和血中细胞因子定量的影响
Xiaoyun Yang1, Trinidad Arceo1, Saloumeh K Fischer1
1BioAnalytical Sciences, Genentech Inc., South San Francisco, CA, USA.
Bioanalysis
|August 12, 2025
概括
血处理延迟显著改变细胞因子水平,只有IL-2Ra保持稳定. 快速的样本处理和受控的储存对于研究中准确的细胞因子生物标志物定量至关重要.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 细胞因子是许多生物过程的重要生物标志物.
- 精确的细胞因子测量是必不可少的,但受到分析前变量的挑战.
- 调查样品处理对细胞因子稳定性的影响对于可靠的结果至关重要.
研究的目的:
- 为了评估血处理和储存温度延迟对八种关键细胞因子量化的影响.
- 为了确定血清和血中不同预分析条件的细胞因子稳定性.
主要方法:
- 来自健康志愿者的血液样本被立即处理或在3,6,24和48小时后处理.
- 样品在室温或4°C下储存.
- 使用多重Ella测定测量细胞因子水平.
主要成果:
- 介素-2受体α链 (IL-2Ra) 是所有测试条件中唯一稳定的细胞因子.
- 观察到其他细胞因子度 (CCL2,CXCL10,IFN-γ,IL-6,IL-7,IL-18,TNFα) 的显著变化,处理延迟.
- 变化早在3小时就显而易见,并在24-48小时内加剧,在4°C的储存中,通常保持水平比室温更好.
结论:
- 延迟的血液处理和储存条件会对细胞因子定量产生重大影响.
- 标准化和快速的样本处理对于准确的细胞因子测量至关重要.
- 必须仔细控制分析前的因素,以确保细胞因子数据在临床和研究环境中的可靠性.
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