一个灵敏的比度温度计,由基于AIEgen的混合兰化物MOF构建,用于细胞内温度测绘
Zhijia Li1,2, Jieping Zhang2, Zhiyuan Wu2
1Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou, Fujian, 350108, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 12, 2025
概括
研究人员开发了一种新的混合胺金属有机框架 (LnMOF),用于精确的细胞下温度传感. 这种聚合诱导排放 (AIE) 材料为活细胞中的细胞内温度测量提供了更高的灵敏度.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物物理学的生物物理.
背景情况:
- 精确的细胞下温度传感对于理解生物过程至关重要.
- 聚合诱导辐射 (AIE) 提供可调节的发光特性.
- 金属有机框架 (MOF) 为功能性材料提供了多功能平台.
研究的目的:
- 开发一种高灵敏度的比度温度计,用于细胞内温度检测.
- 为了合成和描述一种基于AIE的新型混合兰坦化物MOF (LnMOF).
- 为了证明LnMOF在活细胞中的视觉温度计的实际应用.
主要方法:
- 一种基于AIEgen的混合兰坦化物MOF (LnMOF) 的合成.
- 描述LnMOF的光物理特性,包括温度灵敏度.
- 为细胞吸收而对LnMOF进行纳米级修改.
- 在L929和HeLa细胞中的细胞内温度的体外和体内成像.
主要成果:
- 合成的混合LnMOF对生理范围内的温度变化 (291321 K) 具有很高的敏感性.
- 最大相对灵敏度 (Sm) 在321K达到≈7.32%K-1,超过现有的比度发光MOF.
- 在活的L929和HeLa细胞中,成功地实现了细胞内温度的视觉检测.
结论:
- 开发的LnMOF作为一个有效的比度温度计,用于精确的细胞下温度测量.
- 这项工作首次展示了基于MOF的比率温度计在活细胞中实际使用.
- 这些发现为先进的细胞内温度计和生物研究开辟了新的途径.
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