一个protocadherin调解细胞-细胞粘附和完整性口服 placode 在衣Ciona中
bioRxiv : the preprint server for biology
|August 12, 2025
概括
在Ciona robusta中,protocadherin-e (Pcdhe.e) 细胞粘附对于口腔吸管斑点 (OSP) 的发展至关重要. 破坏Pcdhe.e导致OSP结构缺陷,揭示了它在代码完整性中的作用.
科学领域:
- 发育生物学是发展生物学.
- 细胞粘附 细胞粘附
- 编排进化的时间表.
背景情况:
- 斑点是状胚胎中的外皮加厚,形成感官器官.
- 虽然通常被认为是脊椎动物特有的,但在非脊椎动物中可能存在同类的 placodes.
- 在状的Ciona robusta中,口腔吸管 (OSP) 形成了幼虫的口腔吸管.
研究的目的:
- 研究调节Ciona robusta中OSP的形态发生和分离的分子机制.
- 确定细胞-细胞粘附在OSP结构完整性和与周围组织分离中的作用.
主要方法:
- 编辑CRISPR/Cas9基因以破坏OSP细胞中的PCDHE表达.
- 在前面表面外皮中过度表达Pcdhe.e.
- 分析OSP形态和细胞分离的分析.
- 研究Pcdhe.e.的转录调节.
主要成果:
- 在OSP中,Protocadherin-e (Pcdhe.e) 特别得到表达.
- 破坏Pcdhe.e导致OSP结构完整性丧失和物理隔离受损.
- 过度表达Pcdhe.e也破坏了OSP领土形成.
- Pcdhe.e的表达受到Six1/2和Pitx等口腔斑点特异性转录因子的调节.
结论:
- 通过protocadherin介导的细胞-细胞粘附对于Ciona robusta中OSP的结构完整性和分离至关重要.
- 这些发现表明,在状动物中形成斑点的机制得到了保护.
- 同型细胞粘附分子的精确调节对于斑点细胞的发育至关重要.
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