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人类的RPL7和DDX21与HTLV-1Gag相互作用,并增强tRNA^Pro初级化到基因组RNA

Yu-Ci Syu¹, Zixi Long¹, Karin Musier-Forsyth¹

¹Molecular, Cellular, and Developmental Biology Graduate Program, Department of Chemistry and Biochemistry, Center for RNA Biology, and Center for Retrovirus Research, The Ohio State University, Columbus, OH.

bioRxiv : the preprint server for biology

|August 12, 2025

概括

人类T细胞白血病病毒1型 (HTLV-1) 使用细胞辅助因子RPL7和DDX21将原始tRNA化为其基因组RNA. 这种相互作用对HTLV-1逆转录至关重要,并提供潜在的治疗点.

科学领域:

病毒学病毒学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

人类T细胞白血病病毒1型 (HTLV-1) 是一种致癌逆转录病毒.
HTLV-1利用人类的tRNA^Pro进行反向转录 (RT).
在HTLV-1基因组RNA (gRNA) 中,tRNA^Pro与原始结合部位 (PBS) 结合的机制尚未完全理解.

研究的目的:

为了研究HTLV-1 Gag蛋白在tRNA火中的作用.
为了确定促进tRNA^Pro化到HTLV-1 PBS的细胞辅助因子.
为潜在的治疗开发阐明了原始tRNA回火的机制.

主要方法:

净化重组HTLV-1Gag用于初级化试验.
亲和标记/净化质谱 (AP-MS) 用于识别相互作用的蛋白质.
相互共免疫沉 (co-IP) 和域映射研究.
对RPL7和DDX21包装成HTLV-1病毒的分析.

主要成果:

与其域相比,HTLV-1 Gag对tRNA^Pro化具有有限的伴侣活动.
RPL7和DDX21被确定为与HTLV-1 Gag.交互的细胞辅助者.
HTLV-1 Gag通过核酸 (NC) 域中的指与RPL7和DDX21相互作用.
RPL7和DDX21,单独和Gag的协同作用,显著增强tRNA^Pro化到PBS.
RPL7和DDX21被纳入了HTLV-1病毒.

结论:

细胞辅助因子RPL7和DDX21在促进HTLV-1tRNA^Pro原始化中发挥着至关重要的作用.
/RPL7/DDX21复合体对于有效地与gRNA PBS结合的原始素是必不可少的.
了解这些相互作用为开发针对逆转录的新型抗HTLV-1疗法提供了机械洞察力.

关键词:

DDX21 DDX21 是一个字母.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈在 HTLV-1 病毒中,RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 RPL7 反向转录是一种反向转录.在tRNAPro的基础上,我们使用了tRNAPro的初始化.

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