在Streptococcus pneumoniae引起的肺炎期间,肺部营养物质可用性的变化
Hansol Im1,2, Vipin Chembilikandy1, Adonis D'Mello3
1Department of Microbiology, Heersink School of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
肺炎会改变肺部的新陈代谢,从而影响Streptococcus pneumoniae的营养可用性. 细菌基因表达转移,特别是spxB,表明在感染进展过程中适应不断变化的宿主条件.
科学领域:
- 微生物学 微生物学
- 宿主-病原体相互作用
- 代谢学 代谢学 代谢学
背景情况:
- 肺炎链球菌 (Streptococcus pneumoniae) 引起肺炎,但其对呼吸道代谢和细菌适应的影响尚不清楚.
- 了解这些宿主-病原体代谢动态对于开发新疗法至关重要.
研究的目的:
- 在肺炎球菌性肺炎期间全面描述呼吸道代谢场景.
- 研究宿主代谢变化对细菌基因表达和表型的影响.
主要方法:
- 非向的UPLC-ESI-MS/MS代谢学,以分析不同肺炎阶段的呼吸道代谢物.
- 使用66个基因的定制NanoString nCounter面板进行细菌转录组分析.
主要成果:
- 观察到气道代谢物中的动态变化,随着肺炎的进展,最初的增加随后下降.
- 主体代谢变化表明 purin 失调,细胞应激和组织损伤.
- 随着疾病的进展,葡萄糖水平下降,与细菌中spxB基因表达的增加相关,这表明转向混合发酵和更高的过氧化生产.
结论:
- 肺炎球菌性肺炎深刻地改变了肺的代谢环境.
- 代谢变化影响细菌表型,提供了对宿主-病原体相互作用的见解.
- 这种理解可以为针对新陈代谢途径的新型治疗策略提供信息.
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