狗肠道微生物群的有限日常变化:对微生物组研究的含义
David Atuahene1,2, Luke Wolfe2, Oona Elisabet Vanhatalo3
1Department of Veterinary Sciences, School of Agriculture and Veterinary Medicine, University of Turin, Turin, Italy.
Frontiers in veterinary science
|August 12, 2025
概括
单个便样本准确地反映了狗的肠道微生物组,简化了收集. 这项研究发现没有显著的每日变化,验证了对狗肠道微生物群研究的单日采样.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 动物科学动物科学
背景情况:
- 肠道微生物组在狗的健康中起着至关重要的作用,影响消化,免疫和行为.
- 便样本是评估狗肠道微生物群的一种非侵入性方法.
- 目前的协议通常需要连续三天的便收集,以解释所谓的每日变化.
研究的目的:
- 为了确定一天的便样本是否代表了肠道微生物组,与狗的三天收集相比.
- 为了研究狗便微生物组成的潜在每日变化.
- 提供证据,以简化便采样协议在狗微生物组研究.
主要方法:
- 在连续三天内从12只狗身上收集了便样本.
- 将一天样本的微生物组成与三天样本的微生物组成进行了比较.
- 使用了统计分析,包括两个单边等效测试 (TOST) 和PERMANOVA.
主要成果:
- 使用TOST分析,没有发现肠道微生物群的每日显著变化.
- 在PERMANOVA和非度量多维缩放中,在采样日内,微生物组成没有显著差异 (p=0.98).
- 一天的便样本被发现与三天的收集一样具有代表性.
结论:
- 一个便样本足以准确评估狗的肠道微生物群.
- 这些发现支持采用简化单日采样策略进行犬类微生物组研究.
- 这种方法可以显著减少与狗肠道微生物组研究相关的劳动力和成本,而不会影响数据质量.
相关概念视频
Anatomy of the Intestines
74.0K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
74.0K
Bacterial Flora of the Large Intestine
643
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
643
What is Monogastric Digestion?
72.5K
The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
72.5K


