在Saccharomyces cerevisiae中生物合成不规则的单烯
Shengxin Nie1,2,3, Ruiqi Chen1,2,3, Mingyue Ge1,2,3
1State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin, 300072, China.
Synthetic and systems biotechnology
|August 12, 2025
概括
研究人员首次成功地在酵母中生物合成了衣草的关键化合物衣草醇. 在合成生物学中的这一突破实现了创纪录的308.92mg/L的产量,为可持续的工业应用铺平了道路.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 衣草精油中的一种不规则的单烯,拉万杜醇,在化品和费洛蒙配方中具有价值.
- 合成生物学使微生物能够生产有价值的类.
研究的目的:
- 在*Saccharomyces cerevisiae* (酵母菌) 中首次实现拉万杜醇的异质生物合成.
- 为了优化酵母代谢途径,以提高黄醇的生产.
主要方法:
- 选拉万杜利二酸盐合成酶的酵母兼容性.
- 在MVA通路中过度表达关键酶 (IDI1,tHMG,ERG12,ERG8),以促进DMAPP供应.
- 删除竞争性通路基因 (*MLS1*, *CIT2*) 和促进子替代 (*ERG20*) 以减少代谢流失.
- 在5L生物反应器中进行料批发发酵,用于大规模生产.
主要成果:
- 确定了适合异质表达的相容酶.
- 工程酵母菌株实现了136.68 mg/L的瓶头位.
- 在5L生物反应器中,拉万杜醇的最大产量达到308.92 mg/L,这是 *S. cerevisiae* 的新纪录.
结论:
- 在Saccharomyces cerevisiae*中成功建立了第一个兰的异质生物合成.
- 代谢工程策略显著提高了黄醇产量.
- 取得的生产水平表明了工业规模的黄素微生物合成的潜力.
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