Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.5K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exploring neutral backbones in phosphorodiamidate morpholino oligomers: synthesis and biophysical properties.

Chemical communications (Cambridge, England)·2026
Same author

Developmental stage-dependent cellular plasticity governs β-cell reprogramming efficiency in human fibroblasts.

Biochemical and biophysical research communications·2026
Same author

Copy-back RNA synthesis by coronavirus polymerase requires helicase activity and is stimulated by remdesivir and molnupiravir.

Science advances·2026
Same author

Real-World Comparison of Biosimilar Ranibizumab (Ranieyes) and Innovator Ranibizumab (Lucentis/Accentrix) Across Multiple Retinal Vascular Diseases (The BRIO Study).

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Diverse image generation with diffusion models and cross class label learning for polyp classification.

Scientific reports·2026
Same author

Biosimilar anti-vascular endothelial growth factor agents: Clinical outcomes, safety, and cost-effectiveness in India.

Indian journal of ophthalmology·2026

相关实验视频

Updated: Sep 11, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.4K

横切割常见路径数字全息显微镜与代重建.

Harshal Chaudhari, Arnab Das, Harsh Vikram Singh

    Applied optics
    |August 12, 2025
    PubMed
    概括

    一种新的共同路径离轴数字全息显微镜 (DHM) 系统简化了光学设置. 这种先进的DHM方法通过减少异常并提高生物样本的稳定性来增强定量阶段成像.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 生物医学成像技术 生物医学成像技术
    • 显微镜的使用方法

    背景情况:

    • 双路径离轴数字全息显微镜 (DHM) 对定量相位成像非常有价值.
    • 现有的DHM系统面临的挑战包括复杂的光学,偏差和时间不稳定性.

    研究的目的:

    • 提出和验证一个共同路径的离轴DHM系统.
    • 为了克服传统DHM设置的局限性.
    • 为了提高生物样本的相位估计质量.

    主要方法:

    • 一个共同路径离轴的DHM配置,利用对象分散波面的横向剪切.
    • 一个光束分割器将放大对象光束划分为两个侧面剪切的组件.
    • 代算法用于增强相位估计和抑制失焦信息.

    主要成果:

    • 拟议的DHM系统避免了其他侧面剪切方法中常见的图像重复.
    • 使用聚烯珠和HeLa细胞的实验验证证明了有效的成像.
    • 研究证实,与传统的双路DHM相比,时间稳定性得到了改善.

    结论:

    更多相关视频

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.5K

    相关实验视频

    Last Updated: Sep 11, 2025

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.4K
    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.5K
  • 共同路径离轴DHM为定量相位成像提供了简化和更稳定的方法.
  • 代算法有效地提高了相位估计的准确性.
  • 这种技术对先进的生物和材料科学应用有前途.