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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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相关实验视频

Updated: Sep 11, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

通过图像平面旋相口罩在传输测量中改善散射排斥.

Shawn Divitt, K Peter Judd, Matthew B Hart

|August 12, 2025

概括

研究人员使用带有针孔过器的旋转相位面罩,提高了14%的激光发射仪性能. 这种光学方法可以提高信号清晰度,用于在杂介质等散射环境下进行测量.

科学领域:

光学物理学的光学物理.
光子学是一个光子学.
激光技术激光技术激光技术

背景情况:

由于散射,通过的介质测量光传输是具有挑战性的.
信号损失严重影响光学测量在散射环境中的准确性.

研究的目的:

为了提高激光发射仪的信号到背景功率比.
改进散射介质中的光学测量.

主要方法:

增强一个针孔空间过器与旋相口罩和束块.
综合过系统的实验演示.

主要成果:

在信号与背景功率比率上实现了14%的改进.
与单独使用过器相比,通过更长的衰减长度启用成像.

结论:

旋转相口罩和针孔过器的组合有效地减少了散射效应.
这种技术为改善水下和大气应用中的光学测量提供了潜力.

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High-speed Particle Image Velocimetry Near Surfaces

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

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Last Updated: Sep 11, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

High-speed Particle Image Velocimetry Near Surfaces

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014