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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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相关实验视频

Updated: Sep 11, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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基于常见路径配置的紧型倒置数字全息显微镜.

Isma Javed, Muhammad Bilal Hassan, Ramna Khalid

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    概括
    此摘要是机器生成的。

    我们开发了一种新的,紧的3D打印全息显微镜,用于生命科学领域的无标签成像. 这种强大且易于调整的原型简化了现场应用的显微镜.

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    相关实验视频

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    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
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    科学领域:

    • 生命科学 生命科学
    • 显微镜的使用方法
    • 光学工程是指光学工程.

    背景情况:

    • 全息成像提供了微观结构的3D可视化.
    • 目前的显微镜技术 (共聚焦,多光子,数字全息) 有局限性,包括体积庞大的组件,成本和复杂的对齐.
    • 需要更简单,更坚固,更能在现场部署的显微镜解决方案.

    研究的目的:

    • 提出和原型一个紧的,无标签的,易于调整的反转全息显微镜.
    • 克服现有的显微镜技术的局限性.
    • 为了实现实地测量和实时监测活和透明样品.

    主要方法:

    • 开发一个3D打印的反转全息显微镜原型.
    • 使用了通用路径全息配置,与高数值光圈目标兼容.
    • 集成的自动控制和复杂的阶段,用于沿着三个轴的自我聚焦.
    • 杆光场技术用于光学设置简化.

    主要成果:

    • 原型是紧的,没有标签,并且易于对齐.
    • 展示了简单性,坚固性和高时间稳定性.
    • 实现了自动化自我聚焦能力.
    • 能够实时监测活生物和透明样品,而无需使用抗振表.

    结论:

    • 开发的全息显微镜原型解决了当前技术的局限性.
    • 该设备为先进的3D成像提供了一个强大的,独立的,简化的解决方案.
    • 为现场生命科学应用和测量开辟了新的可能性.