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Updated: Sep 11, 2025

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
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改善了人类乳头瘤病毒驱动的头癌细胞系稳定生成的方法

Phuong N Le1, Stephen B Keysar1, Bettina Miller1

  • 1Division of Medical Oncology, Department of Medicine, University of Colorado School of Medicine (UCSOM), Aurora, Colorado, USA.

Molecular carcinogenesis
|August 12, 2025
PubMed
概括

研究人员开发了两个改进的协议,以建立稳定的细胞系,这对于癌症研究至关重要. 这些方法利用光激活细胞分类 (FAC) 和源细胞共同培养来克服创造新癌症细胞系的挑战.

关键词:
细胞培养培养的细胞培养.细胞系细胞系的细胞系.料细胞是一种料细胞.

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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