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帕拉梅分泌中的酸是一种高效的定数感应诱导剂

Xingyao Ye^1,2, Xiaojun Niu^1,2,3, Dongqing Zhang²

¹School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.

The ISME journal

|August 12, 2025

概括

由原生动物分泌的酸 (CA) 作为细菌信号分子,影响定数感应并增强anammox过程中的去除. 这一发现揭示了物种间的沟通,跨越不同的食物营养层.

科学领域:

微生物学微生物学
环境科学环境科学
生态生态学生态生态学

背景情况:

质量感应 (QS) 是细菌细胞对细胞的通信,调节基于人口密度的集体行为.
在多个物种群体中,特别是捕食者群体中,QS的生态作用尚不清楚.
N-类同类素乳 (AHLs) 是像Pseudomonas aeruginosa这样的格兰阴性细菌中常见的自诱导体.

研究的目的:

研究原生分泌物酸 (CA) 对 Pseudomonas aeruginosa PAO1.1 中的定数感应的影响.
探索CA在提高anammox过程的启动和效率方面的潜在应用.

主要方法:

研究了外源CA与Pseudomonas aeruginosa PAO1.1.中的LuxR型定数感知调节器 (LasR/RhlR) 的相互作用.
测试了CA对AHL合成缺陷的Pseudomonas aeruginosa突变 (PAO1-ΔlasI/ΔrhlI) 的影响.
应用CA加速anammox过程,并量化其对细菌丰度和去除率的影响.

主要成果:

酸 (CA) 作为AHLs的功能模拟物,激活LasR/RhlR调节器并恢复突变菌株中的定数感应.
CA显著增强了anammox细菌联盟的聚合和动态.
用500nMCA治疗增加了76.3%的阿纳莫克斯细菌丰度,并实现了高的去除率 (1.42g N·L-1·d-1).

结论:

酸调解原生动物和细菌之间的物种间通信,影响细菌的定数感应.
可以利用CA优化无氧氨氧化 (anammox) 生物反应堆的启动和性能.
这项研究突出了水生微生物群落中交叉营养水平信号的新机制.

关键词:

帕拉梅是其中一种.在 anammox 中使用.氨酸是氨酸中的一种.议员数量检测是指对议员数量进行检测.

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