使用cFOOT-seqq对转录因子占用率和动态的全基因组调查
Heng Wang1,2, Ang Wu1, Meng-Chen Yang1
1Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of the Ministry of Education, Department of Orthopedics, Tongji Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200065, China.
Protein & cell
|August 12, 2025
概括
我们开发了cFOOT-seq,这是一种检测跨基因组转录因子 (TF) 结合的新方法. 这种方法精确地绘制了TF占用在开放和关闭的染色质,即使有有限的细胞.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基因调节取决于转录因子 (TF) 与调节性DNA元素结合.
- 同时检测大量的全基因组TF是一个重大的技术挑战.
- 了解TF结合动态对于破译基因调节至关重要.
研究的目的:
- 开发一种高分辨率的定量方法,用于全基因组TF绑定评估.
- 为了使TF足迹在开放和关闭的染色体区域.
- 为了促进TF占用动态和cis-regulatory架构的研究.
主要方法:
- 开发了cFOOT-seq,一种基于cytosine deaminase的TF足迹测定方法,使用SsdAtox.
- 将可访问的细胞因子转化为乌拉,保持基因组完整性.
- 结合cFOOT-seq与ATAC-seq用于敏感的单分子单细胞TF占用检测.
主要成果:
- 启用了TF足迹的划分和TF占用基因组范围的量化.
- 在开放和关闭的染色质中评估了TF结合,即使细胞数量很小.
- 使用FootTrack分析证明了TF绑定站点的 de novo预测和TF占用动态的跟踪.
结论:
- cFOOT-seq为调查全基因组TF占用动态提供了一个强大的方法.
- 该方法阐明了基因调节背后的 cis 调节体系结构.
- cFOOT-seq捕捉了细胞类型特定的TFs,重编程期间的TF动态,以及TF对染色体重塑剂的依赖.
关键词:
这就是BRM01414的意思.在TF足迹中,TF足迹是什么?TF 占用率 在 TF 占用率.TF组织是TF组织.染色质可访问性 染色质可访问性染色体的景观 染色体的景观染色体重塑 染色体重塑的方法基因调节 基因调节 基因调节核的位置是核的位置.转录因子的转录因子更多相关视频
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