相关实验视频

Updated: Jun 8, 2026

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
14:46

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Published on: May 28, 2015

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凸型ML:在不可逆转突变模型下快速准确地估计分支长度,通过应用CRISPR/Cas9基谱系追踪来说明

Sebastian Prillo1, Akshay Ravoor1, Nir Yosef1,2

  • 1Computer Science Division, University of California, Berkeley, USA.

Systematic biology
|August 12, 2025
PubMed
概括

我们介绍了ConvexML,这是一种使用不可逆转突变模型估计家族遗传树枝长度的新方法,对于CRISPR/Cas9谱系追踪至关重要. 这种方法将细胞谱系树精细化为时间解析的时间表,为细胞群体动态提供了洞察力.

关键词:
这就是CRISPR/Cas9的作用.最大概率估计,节,凸起优化,单细胞血统追踪,分支长度估计,不可逆转的突变模型,伪计数.

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells10:07

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CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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