开发和验证"稀释和射击"LC-MS/MS在尿中的方法,适用于临床环境中的查和确认分析
Dimitra Florou1, Amvrosios Orfanidis2, Vassiliki A Boumba2
1Department of Forensic Medicine and Toxicology, Faculty of Medicine, School of Health Sciences, University of Ioannina, University Campus, Ioannina, 45500, Greece.
概括
这项研究开发了一种经过验证的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于检测尿液中的115种药物. 可靠的稀释和射击方法有助于临床和法医毒理学,包括监测康复计划.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
- 临床化学 临床化学
背景情况:
- 尿液毒理分析对于临床诊断,法医调查和物质使用监测至关重要.
- 稀释和射击方法与LC-MS/MS相结合,为药物检测提供了一种有效和可靠的方法.
研究的目的:
- 开发和验证一个全面的LC-MS/MS方法,用于同时检测和定量115种药物和尿液中的代谢物.
- 为各种毒理学应用建立一个强大的分析工具.
主要方法:
- 使用甲醇:乙二混合物的稀释和射击样本准备技术得到了优化.
- 在C18柱上采用液体染色学-双重质谱法 (LC-MS/MS),在7.5分钟内进行梯度化.
- 方法验证包括对选择性,灵敏性,恢复性,准确性,精度和稳定性的评估.
主要成果:
- 该方法实现了高灵敏度,检测极限 (LOD) 和量化极限 (LOQ) 分别在0.01~1.5 ng/mL和0.05~5 ng/mL之间.
- 经过验证的方法在所有验证参数上都表现出令人满意的性能.
- 这种方法成功地被应用到12周的康复计划中对个体的尿样进行监测.
结论:
- 开发的LC-MS/MS方法具有选择性,灵敏性和可靠性,可同时分析尿液中的各种药物和代谢物.
- 这种方法是临床和法医毒理学,药物犯罪调查和药物滥用监测的宝贵工具.
- 在康复计划中的应用凸显了其在追踪处方药和非法物质使用中的实用性.
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