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衰退的美国大黄蜂,Bombus pensylvanicus的新参考基因组组合

Jeffrey D Lozier¹, Rena M Schweizer^2,3, Sheina B Sim⁴

¹Department of Biological Sciences, The University of Alabama, Tuscaloosa, AL 35487, USA.

G3 (Bethesda, Md.)

|August 12, 2025

概括

我们生成了第一个染色体水平的基因组组合,用于正在衰退的黄蜂,Bombus pensylvanicus. 这种基础资源将有助于研究大黄蜂的衰退和遗传变异.

科学领域:

基因组学就是基因组学.
昆虫学昆虫学是一门学科.
保护生物学保护生物学

背景情况:

本地授粉者Bombus pensylvanicus已经经历了显著的范围和丰度下降.
了解遗传变异对于解决大黄蜂减少问题至关重要.

研究的目的:

为了呈现第一个染色体层次的基因组组装Bombus pensylvanicus.
为研究大黄蜂的衰退和进化提供一个有价值的基因组资源.

主要方法:

基因组组装使用太平洋生物科学单分子HiFi序列和Hi-C数据.
用真核生物基因组注释管道进行注释 - 外部 (EGAPx) 和RNA测序.
用于基因组完整性评估的BUSCO分析.

主要成果:

一个染色体级的基因组组合大约352.6Mb与19个主要的伪分子.
鉴定了11,411个基因,具有高基因组和蛋白质完整性 (分别为98.5%和99.0%).
与其他Bombus基因组进行合成分析,检查重复元素分布.

结论:

B. pensylvanicus基因组组合是未来研究的高质量资源.
这种基因组将促进对大黄蜂衰退的遗传基础的研究.
比较基因组学可以揭示Bombus属内的进化见解.

关键词:

蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂,蜜蜂.生物多样性生物多样性蜂群中的大黄蜂,就是大黄蜂.保护保护保护基因组组装组合的基因组.其他授粉者授粉者

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Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.