相关实验视频

Updated: Sep 11, 2025

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

解读由Musashi-1进行的RNA识别,以设计用于体外和体内应用的蛋白质和RNA变体

Anna Pérez-Ràfols^1,2, Guillermo Pérez-Ropero^3,4, Linda Cerofolini¹

¹Magnetic Resonance Center (CERM) and Department of Chemistry, University of Florence, and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine (CIRMMP), Sesto Fiorentino 50019, FI, Italy.

Nucleic acids research

|August 12, 2025

概括

研究人员使用计算和结构方法探索了人类Musashi-1 (MSI-1) 蛋白与RNA的相互作用. 这项研究增强了对MSI-1RNA结合的理解,为新的生物分子开发铺平了道路.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
生物信息学是一种生物信息学.

背景情况:

人类Musashi-1 (MSI-1) 是一种RNA结合蛋白,对于调节RNA命运至关重要.
MSI-1使用两个RNA识别动机 (RRM) 域来结合特定的RNA序列,如 (G/A) U1-3AGU和UAG.

研究的目的:

分析MSI-1的RRM域与单链和结构RNA连接物的结合.
使用计算工具设计蛋白质和RNA修饰,以调节结合亲和力和选择性.
调查MSI-1的RRM域之间对RNA结合位点的竞争.

主要方法:

结合结构生物学和计算方法.
使用RRMScorer计算工具来设计蛋白质和RNA的替代.
使用核磁共振 (NMR) 和表面等离子体共振 (SPR) 进行实验验证.

主要成果:

成功预测并通过实验验证设计的蛋白质-RNA相互作用.
研究了MSI-1在线性RNA和harpinRNA上的两个RRM域之间的结合竞争.
为MSI-1-RNA相互作用的复杂性提供了洞察力.

结论:

这项研究阐明了MSI-1与RNA配体相互作用的关键方面.
这些发现为开发用于研究和治疗应用的新生物分子铺平了道路.
了解MSI-1RNA结合对于推动基于RNA的生物技术至关重要.

更多相关视频

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

相关概念视频

Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...