相关实验视频

Updated: Sep 11, 2025

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

简单的分子方法的评估,以区分新出现的皮肤植物Trichophyton indotineae

Shima Aboutalebian^1,2, Zahra Jahanshiri³, Mohammad Reza Shidfar⁴

¹Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Medical mycology

|August 12, 2025

概括

准确识别Trichophyton indotineae对于治疗耐药真菌感染至关重要. 新的分子方法,ITS-PCR-RFLP和拓酶II-PCR-RFLP,提供了一种快速,负担得起和可靠的方法,在临床实验室中区分这种病原体.

科学领域:

医学真菌学医学真菌学
分子诊断学分子诊断
抗微生物耐药性抗微生物耐药性

背景情况:

三菌 (Trichophyton indotineae) 是一个日益严重的全球健康问题,导致难以治疗的皮肤感染和治疗失败.
准确的鉴定对于有效的治疗和控制抗真菌耐药性的传播至关重要.
目前的诊断方法很难从类似的真菌中可靠地区分T. indotineae.

研究的目的:

开发和评估简单,具有成本效益的分子方法,以准确区分T. indotineae.
为了比较PCR-RFLP和特定PCR试验的疗效,用于识别T. indotineae.

主要方法:

在基分析中,在ITS和topoisomerase II基因中找到特定的酶切割点.
从430个皮肤植物分离物中PCR放大ITS和topoisomerase II基因.
使用EarI和BsuRI酶进行限制片长多态化 (PCR-RFLP) 分析,并评估T. indotineae特异性的PCR测定.

主要成果:

ITS-PCR-RFLP (EarI) 和拓酶II-PCR-RFLP (BsuRI) 均准确地区分了T. indotineae,显示出高灵敏度和特异性.
以前识别的分离物被新PCR-RFLP方法正确地分类.
特定于T. indotineae的PCR测定具有高度灵敏性,但与T. tonsurans.显示了轻微的交叉反应性.

结论:

ITS-PCR-RFLP和拓酶II-PCR-RFLP是T. indotineae鉴定的准确,负担得起和快速的方法.
这些方法适用于临床实验室的常规使用,特别是在资源有限的环境中.
由于潜在的交叉反应性,T. indotineae特定的PCR测定需要谨慎的解释.

关键词:

这种植物是Trichophyton indotineae.一个ITS区域的ITS区域.这是PCR-RFLP.皮肤细胞 (dermatophytes) 是一种细胞.分子识别分子识别限制酶限制酶限制酶特定物种的原料原料.托波色马酶II基因被发现

更多相关视频

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers

Published on: September 29, 2023

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

Published on: October 28, 2021

相关概念视频

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...