糖尿病引起的新陈代谢和免疫活性变化的PET成像
Shannon E Lynch1,2, Heba M Alsheikh3, Patrick N Song1,2
1Department of Radiology, The University of Alabama at Birmingham, VH G082, 1670 University Blvd, Birmingham, AL, 35233, USA.
Molecular imaging and biology
|August 12, 2025
概括
肥胖和2型糖尿病 (T2D) 改变免疫反应和葡萄糖代谢. 分子成像显示,糖尿病肥胖小鼠的瘤免疫力受到抑制,影响瘤微环境和系统器官.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 代谢疾病 代谢疾病
- 分子成像学分子成像学
背景情况:
- 肥胖和2型糖尿病 (T2D) 通过改变葡萄糖代谢,显著影响瘤微环境,可能阻碍免疫细胞功能.
- 定子发射断层扫描 (PET) 提供了一种非侵入性方法,用于可视化和量化瘤和全身器官内的免疫细胞群.
研究的目的:
- 采用先进的分子成像技术,在肥胖和T2D的小鼠模型中定量评估系统和瘤特异性免疫反应.
- 用PET成像研究肥胖和T2D对葡萄糖代谢和免疫细胞透/激活的影响.
主要方法:
- 高脂肪饮食 (HFD) 和链毒素用于诱导C57BL6/J小鼠的肥胖和T2D.
- 使用葡萄糖代谢 ([18F]-FDG),M2巨细胞 ([68Ga]Ga-RP832c),效应细胞激活 (granzyme B, [68Ga]Ga-GZP) 和神经炎症 ([18F]-DPA-714) 的标记物进行了PET成像.
- 在各种器官和瘤中分析了标准化吸收值 (SUV),随后进行流细胞计,以进行详细的免疫细胞表征.
主要成果:
- 患有糖尿病的肥胖小鼠表现出葡萄糖代谢的改变,大脑和棕色脂肪组织 (BAT) 的PET标记物吸收减少,但瘤没有.
- 系统性免疫分析显示,在多个器官 (包括肌肉,心脏和大脑) 中,M2巨细胞和大酶B信号增加.
- 瘤分析显示CD206+巨细胞显著增加,而表达大酶B的CD8+T细胞没有显著变化.
结论:
- PET成像表明,肥胖和T2D深刻地改变了全身葡萄糖代谢和免疫激活模式.
- 这些代谢和免疫变化与糖尿病肥胖小鼠瘤微环境中的瘤免疫激活抑制有关.
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