单细胞和批量转录组分析揭示了子宫癌中RNA结合蛋白调节程序
Shasha Yang1, Jingjing Zeng2, Yong Wang1
1Department of Obstetrics and Gynecology, First Affiliated Hospital of Guangxi Medical University, Nanning City, 530000, Guangxi Zhuang Autonomous Region, China.
Scientific reports
|August 13, 2025
概括
RNA结合蛋白 (RBPs) 在宫癌 (CESC) 中表现出不同的模式. 异常的RBP表达和拼接事件与CESC进展有关,为HPV阳性CESC提供潜在的诊断和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- RNA结合蛋白 (RBPs) 的异常表达与各种疾病有关.
- 在宫平细胞癌和宫内腺癌 (CESC) 中,RBPs的单细胞格局尚不清楚.
- 人类乳头瘤病毒 (HPV) 状态显著影响CESC的发展和进展.
研究的目的:
- 调查RBPs在HPV阳性和HPV阴性CESC中的单细胞表达模式.
- 确定与CESC进展相关的RBPs和RNA替代拼接事件.
- 评估RBPs在CESC中的预后意义.
主要方法:
- 对CESC样本的单细胞RNA测序分析.
- 使用SUVA软件进行差异基因表达和替代拼接分析.
- 免疫组织化学验证RBP表达和与患者预后的相关性.
主要成果:
- 阳性HPV的CESC样本显示了上皮细胞的比例增加.
- 在HPV阳性和HPV阴性CESC之间,RBP表达异质,上皮细胞表现出不同的RBP.
- 四个RBPs (CSTB,TIPARP,NDRG1,NDRG2) 与25个RNA替代拼接事件相关;TIPARP在HPV阳性CESC中下调.
- 结合RBPs,阶段和治疗的预后模型显示了潜在的临床实用性.
结论:
- 在HPV阳性与HPV阴性CESC中,RBPs表现出不同的表达模式.
- RNA拼接事件与CESC进展有显著的关联.
- RBPs代表了HPV阳性CESC检测和治疗的潜在生物标志物和治疗点.
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