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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Light Acquisition02:16

Light Acquisition

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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基于多尺度总概括变化的单射结构光照明.

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    这项研究引入了一种使用多尺度总概括变化的新单拍摄3D重建方法. 它通过减少光谱泄漏和频率异化来显著提高纹理表面的精度.

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    科学领域:

    • 计算机视觉 计算机视觉
    • 光学计量学 在光学计量学

    背景情况:

    • 单拍3D重建对于高速应用至关重要.
    • 由于光谱泄漏和别名化,里埃变换造型测量与纹理表面作斗争.

    研究的目的:

    • 为复杂的表面开发一个强大的单射3D重建框架.
    • 通过解决常规里叶变换概况测量的局限性来提高准确性.

    主要方法:

    • 提出了一个使用多尺度总的通用变化和变化优化的框架.
    • 集成的多频边缘调制,以产生双频异构模式.
    • 采用了适应性导向总的通用变化模型用于边缘部件提取.

    主要成果:

    • 在高度纹理的表面上实现了卓越的重建质量.
    • 与传统方法相比,证明了增强的稳定性.
    • 通过使用双频异质素技术有效抑制光谱重叠.

    结论:

    • 拟议的方法在对具有挑战性的表面进行一次性3D重建方面取得了重大进展.
    • 多尺度总的通用变化有效地处理复杂的纹理.
    • 该框架为高速3D成像提供了更准确,更强大的解决方案.