对定量斜背照明显微镜 (qOBM) 的光学转移函数的实验评估
Optics express
|August 13, 2025
概括
定量斜背照明显微镜 (qOBM) 现在可以实验性地测量厚组织中的光散射. 这一进步改善了各种生物样本的3D成像和折射率断层扫描.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜的使用方法
- 定量成像技术 定量成像
背景情况:
- 定量斜背照明显微镜 (qOBM) 是一种用于散射样品的3D成像技术.
- 精确的相位和折射率 (RI) 量化需要了解样本内的光的角度分布.
- 目前的方法依赖于蒙特卡洛模拟,受预定义的散射特性限制.
研究的目的:
- 开发和验证一种用于测量在厚散射介质中多次散射光的角分布的实验方法.
- 克服量化阶段和RI成像中的模拟依赖方法的局限性.
- 提高qOBM对更广泛的生物组织的适用性,包括那些具有未知的分散特征的生物组织.
主要方法:
- 开发了一种新的实验方法,直接测量成像平面上的散射光的角分布.
- 将该方法应用于各种生物样本:切除的大脑组织,活体皮肤和FFPE组织.
- 通过实验获得的角光分布数据验证了qOBM的定量忠实性.
主要成果:
- 成功测量了多重散射光在各种生物组织中的角分布.
- 与基于模拟的方法相比,实验测量提高了qOBM的准确性和可靠性.
- 在不同样本类型中确认了高准确度定量阶段和RI断层扫描.
结论:
- 对光角分布的直接实验测量显著提高了qOBM的能力.
- 这种新的方法扩大了qOBM对具有未知或可变散射特性的复杂样品的实用性.
- 该方法为生物组织的定量3D成像提供了更强大的基础.
更多相关视频
06:55Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
Published on: June 6, 2017
7.7K
12:54Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
3.4K
相关概念视频
Imaging Biological Samples with Optical Microscopy
5.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.3K
Phase Contrast and Differential Interference Contrast Microscopy
9.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.5K
Total Internal Reflection Fluorescence Microscopy
6.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.6K
Confocal Fluorescence Microscopy
14.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.3K
