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PagBTB74 在Populus alba × Populus glandulosa中的木材形成过程中负面调节二级壁生物合成

Jing Yue¹, Linxi Yang¹, Shouling Xu²

¹State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.

Plant, cell & environment

|August 13, 2025

概括

这项研究表明,BTB/POZ蛋白PagBTB74在Populus中木材形成过程中作为二级细胞壁 (SCW) 生物合成的负调节剂. 它直接抑制了关键的SCW生物合成基因,影响了木材的发展.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
林业林业林业林业林业

背景情况:

二次细胞壁 (SCW) 合成对于木材形成至关重要.
已知NAC和MYB转录因子是SCW调节者.
BTB/POZ蛋白在木材形成中的作用尚未被探索.

研究的目的:

研究BTB/POZ蛋白,PagBTB74在Populus alba × Populus glandulosa中的木材形成中的功能.
确定PagBTB74在二次细胞壁生物合成中的调节机制.

主要方法:

生成的转基因Populus植物具有PagBTB74过度表达,主导抑制和RNAi沉默.
使用了染色体免疫沉 (ChIP),RNA测序 (RNA-seq) 和酵母一杂化 (Y1H).
进行了效应器-报告器测试以评估转录活性.

主要成果:

转基因植物表现出异构体宽度和SCW厚度的改变,PagBTB74影响了这些特征.
帕格BTB74直接准并降低关键的SCW生物合成基因的调节:帕格VND6-C1,帕格CesA7和帕格XTH4.
PagBTB74表现出转录抑制活动.

结论:

这项研究是第一个确定BTB转录因子作为木材形成中SCW生物合成的负调节者.
PagBTB74通过直接抑制参与该过程的基本基因来负面调节SCW生物合成.

关键词:

这是一种BTB/POZ蛋白质.页面BTB74 页面BTB74 页面这是一棵树,树.二次细胞壁的生物合成.二次墙壁的加厚使其变厚.转录因子的转录因子形成木材的树木形成.

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