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底层膜的泛癌景观:多组学研究和单细胞测序验证
Yi Tang1,2, Qiuhuan Zhang1,3, Zhuoer Yuan4
1Graduate school, Jinan University, Guangzhou, People's Republic of China.
在癌症中基底膜 (BM) 改造与免疫逃避有关. BM调节基因的变化与免疫检查点和瘤免疫功能障碍相关,这表明BM积极影响癌症.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 皮质癌细胞必须穿透底层膜 (BM) 进行转移.
- 脑细胞作为一个屏障,防止癌细胞的入侵和调节内细胞/外细胞.
- 了解BM在瘤免疫力中的作用对于开发抗癌策略至关重要.
研究的目的:
- 研究BM调节基因,遗传变异和瘤免疫之间的关系.
- 为了确定BM重塑是否影响免疫检查点和瘤免疫功能障碍.
- 为了分析各种癌症类型的BM变化.
主要方法:
- 对33种癌症类型的癌症基因图谱 (TCGA) 基因表达数据的分析.
- 对BM调节基因,拷贝数变异,免疫基因和瘤免疫功能障碍和排斥 (TIDE) 评分的相关性分析.
- 免疫组织化学,单个样本基因组丰富分析 (ssGSEA) 用于BM得分计算,以及单细胞RNA测序.
主要成果:
- 乳腺癌调节基因 (BMG) 在癌症类型中显示出显著的异质性,在瘤中最多是上调调的.
- 常见的BMG遗传变异包括误解突变,异构缺失和放大.
- 在许多癌症中,观察到BM得分 (BMS),免疫检查点 (CD276,NRP1,C10orf54) 和TIDE得分之间的正相关性.
结论:
- BM调节基因以癌症特异的方式发生变化.
- 这些BM变化与免疫检查点表达和免疫功能障碍有关.
- 底层膜重塑积极调节瘤微环境中的免疫抵抗.
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