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相关概念视频

Leaky Scanning02:28

Leaky Scanning

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Improving Translational Accuracy02:07

Improving Translational Accuracy

11.9K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
11.9K
Translational Regulation01:29

Translational Regulation

93
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
93
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

23.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.4K
Initiation of Translation02:33

Initiation of Translation

34.5K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
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相关实验视频

Updated: Sep 11, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

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解码circRNA翻译:计算方法开发中的挑战和进步

Jingjing Zhang1,2, Rui Zhou1,2, Huiling Zhang3

  • 1Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Frontiers in genetics
|August 13, 2025
PubMed
概括
此摘要是机器生成的。

循环RNAs (circRNAs) 可以编码蛋白质,使得计算工具对于研究它们的翻译至关重要. 本综述对这些工具进行了基准评估,发现cirCodAn有效,并强调了培训数据的重要性.

关键词:
生物信息学是一种生物信息学.环RNA 环RNA 是一个环RNA.编码潜在的编码潜力功能 功能 功能 功能.翻译翻译翻译翻译翻译翻译

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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Quantification of Circular RNAs Using Digital Droplet PCR
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Quantification of Circular RNAs Using Digital Droplet PCR

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相关实验视频

Last Updated: Sep 11, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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Quantification of Circular RNAs Using Digital Droplet PCR
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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 循环RNAs (circRNAs) 越来越多地被认为是它们在编码功能性蛋白质中的角色.
  • 由于其复杂性,计算方法对于分析circRNA翻译至关重要.
  • 了解circRNA生物发生和翻译机制是开发有效分析工具的关键.

研究的目的:

  • 为 circRNA 翻译分析提供计算方法的全面审查.
  • 系统地引入现有工具,它们的算法和支持资源.
  • 用统一的数据集对基于序列的方法的性能进行基准测试.

主要方法:

  • 关于circRNA生物发生,翻译,实验技术和数据库的文献综述.
  • 系统介绍和分类用于circRNA翻译分析的计算工具.
  • 使用标准化数据集对基于序列的方法的性能比较.

主要成果:

  • cirCodAn在评估的工具中表现出卓越的预测准确性和用户可访问性.
  • 训练数据的选择显著影响circRNA转换计算模型的性能.
  • 现有的实验技术和数据库为计算方法的开发提供了至关重要的支持.

结论:

  • 本综述为选择和应用circRNA翻译分析工具提供了有价值的参考.
  • 它为在这个领域开发和改进未来的计算策略提供了必要的指导.
  • 对circRNA转化进行准确的计算分析对于推进分子生物学研究至关重要.