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在人类表皮质细胞模型中,内分泌酶体系统驱动着光的形成

Anusha Rengarajan¹, Sicong Wang¹, Chien-Wei Lin²

¹Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA.

bioRxiv : the preprint server for biology

|August 13, 2025

概括

这项研究揭示了控制人类表皮质细胞光线形成的新型内解体机制. RAB35 调节 podocalyxin (PODXL) 贩运,确保在早期胚胎植入期间适当的 apicosome 发展.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
干细胞生物学干细胞生物学

背景情况:

在人类胚胎植入过程中,表皮质光的形成至关重要.
对这个过程来说,内溶性体贩运至关重要,但关键的调节者是未知的.
基体形成代表了光发育的最早阶段.

研究的目的:

在人类表皮质细胞发育过程中,研究在表皮质细胞形成期间的内溶酶体动力学.
在此背景下,确定控制波多卡利辛 (PODXL) 走私的分子参与者.
阐明角膜形态发生的新机制.

主要方法:

利用人类多能干细胞模型用于表皮质细胞的发育.
具有特征的混合早期/晚期内分泌体和晚期内分泌体/溶解体动态.
采用基于APEX2的空间蛋白质组学来识别PODXL相互作用的蛋白质.

主要成果:

在PODXL贩运中发现了一种混合的早期/晚期内分泌体和独特的晚期内分泌体/溶解体动态.
确定RAB35作为PODXL流量的关键调节者和类细胞形成.
RAB35促进了内体过渡和 lysosome 形成,限制了过量的 apicosomes.

结论:

在人类表皮质细胞形成过程中,新型的内溶体机制控制着状膜形态发生.
RAB35在调节PODXL水平方面发挥着至关重要的作用,以确保虫体的正常发育.
这项研究为早期人类胚胎发育提供了分子洞察力.

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