Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluating the Combined Effects of Elevated [CO<sub>2</sub>] and Accelerated NPQ Relaxation on Yield, Physiology and Transcription in Soybean.

Plant, cell & environment·2026
Same author

A Fluorescence-Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants.

Plant direct·2026
Same author

Making conferences in the plant sciences more inclusive through community recommendations.

eLife·2025
Same author

Phylogenetic diversity of light-dependent phosphorylation of Thr78 in Rubisco activase.

Journal of experimental botany·2025
Same author

Editorial: How SynBio can *realistically* impact crop improvement and agriculture.

Plant physiology·2025
Same author

Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.

The Plant cell·2025

相关实验视频

Updated: Jun 12, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.7K

黄金大门兼容的系统,用于在EEEEEEEEEEEEEEEEEEEEEEE的连续定向进化. 大肠杆菌

Ignacio Sparrow Muñoz1,2,3, Steven J Burgess1,2,3

  • 1Department of Plant Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.

Synthetic biology (Oxford, England)
|August 13, 2025
PubMed
概括

研究人员开发了一套模块化工具包,用于使用脱氨酶融合的病毒RNA聚合酶进行连续定向进化. 这种灵活的系统可以通过增强序列空间探索,实现高效的蛋白质工程和发现新型基因功能.

关键词:
黄金大门是黄金大门的一个门.持续的定向进化是一个持续的定向进化.合成生物学 合成生物学工具包是一个工具包.

更多相关视频

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.0K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.4K

相关实验视频

Last Updated: Jun 12, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.7K
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

11.0K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.4K

科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 定向进化是一种强大的蛋白质工程方法,它探索基因变异以改善功能.
  • 持续的定向进化通过将超变异与单个细胞内的选择联系起来来增强这一过程.
  • 现有的方法可能是不灵活和劳动密集型的,限制了它们的广泛应用.

研究的目的:

  • 开发一个通用和可访问的模块化工具包,用于持续的定向进化.
  • 为了适应病毒RNA聚合酶的基因特异性突变和功能选择.
  • 为了使研究人员能够为各种进化运动构建定制等离子体.

主要方法:

  • 使用金门组件进行模块化等离子体构造.
  • 开发了一套具有deaminase融合病毒RNA聚合酶的工具包.
  • 结合了来自菌体SP6的替代RNA聚合酶来证明多功能性.

主要成果:

  • 该工具包促进了持续的定向进化,提高了可访问性和多功能性.
  • 使用工程RNA聚合酶证明了基因特异性突变的引入.
  • 成功地为复杂的进化策略建造了定制塑体.

结论:

  • 本文所介绍的工具包显著提高了持续定向进化的方便性和灵活性.
  • 这一进步扩大了合成生物学工具箱用于蛋白质工程.
  • 能够进行更广泛的研究,发现新的基因功能和优化蛋白质性能.