由Se兴奋剂诱导的结构波动增加了Ru单原子纳米酶的氧化酶类活性,用于生物传感
Jianping Guan1, Jinhua Hu1, Xu Liu1
1Department of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Analytical chemistry
|August 13, 2025
概括
研究人员在化碳 (RuSe-N/C) 催化剂上开发了Ru-Se双单原子位点. 这些催化剂表现出增强的过氧化酶类活性,为敏感的生物传感应用铺平了道路.
科学领域:
- 材料科学 材料科学 材料科学
- 催化剂是一种催化剂.
- 纳米技术纳米技术
背景情况:
- 自然酶由于其特定的结构而表现出高的催化活性.
- 用人工催化剂模仿酶结构是材料科学的一个关键目标.
- 单原子催化剂为各种应用提供独特的特性.
研究的目的:
- 用于制造Ru-Se在添加碳 (RuSe-N/C) 催化剂上的双单原子位点.
- 为了研究RuSe-N/C.的增强的过氧化酶类活性.
- 开发一种双模式生物传感平台,用于性酸酶 (ALP) 活性检测.
主要方法:
- 原子捕获策略用于制造Ru-Se在添加碳的双单原子位点上.
- 催化剂结构和组成的表征.
- 密度函数计算 (DFT) 来理解催化机制.
- 开发色度和光热生物传感平台.
主要成果:
- 与Ru-N/C (SA = 8.3 U mg-1) 相比,RuSe-N/C催化剂表现出明显增强的过氧化酶类活性 (SA = 94.3 U mg-1).
- 据DFT的计算显示,Se兴奋剂诱导结构扭曲,并促进Ru原子的电荷积累,增强H2O2和OH吸附.
- 一个双模式生物传感平台实现了ALP活动的低检测极限 (0.0102 mU mL−1色度,0.0738 mU mL−1光热).
结论:
- 制造的RuSe-N/C催化剂表现出优异的过氧化酶类活性,这归因于Se兴奋剂诱导的结构变化.
- 开发的基于RuSe-N/C的生物传感平台为ALP提供了高灵敏度和双模式检测能力.
- 这项研究提出了设计高效单原子纳米酶 (SAzymes) 用于生物传感应用的新策略.
更多相关视频
10:43Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
Published on: July 21, 2023
3.5K
10:59Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
Published on: May 12, 2023
2.9K
相关概念视频
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Nuclear Overhauser Enhancement (NOE)
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
