生物污泥中的晶体沉:尿素和化使用非生长的Sporosarcina pasteurii的影响
Narjes Gandali Mostafa1, Gholam Reza Ghezelbash1, Mohammad Shafiei1
1Department of Biology, Faculty of Science, Shahid Chamaran University of Ahvaz, Ahvaz 61357-83151, Iran.
FEMS microbiology letters
|August 13, 2025
概括
不生长的细菌有效地沉石,这是生物泥应用中至关重要的矿物质. 最佳条件 (0.3M尿素和化) 产生高纯度的石晶体,颗粒大小可控.
科学领域:
- 生物矿物化 生物矿物化
- 材料科学 材料科学 材料科学
- 微生物学 微生物学
背景情况:
- 不生长的细菌细胞的石沉速度比生长的细胞要快.
- 菌体 (Sporosarcina pasteurii) 是研究生物矿物化的模型生物.
- 生物污泥在混凝土和土壤增强方面具有潜在的应用.
研究的目的:
- 为了研究尿素和化度对非生长的Sporosarcina pasteurii石沉的影响.
- 确定最佳的反应剂度,以最大限度地提高石产量和纯度.
- 分析沉物的晶体类型和颗粒大小分布.
主要方法:
- 细菌细胞 (Sporosarcina pasteurii) 被收集并重新悬浮.
- 悬浮液与不同度的尿素和化溶液混合.
- 使用X射线衍射 (XRD),能量分散式X射线光谱,场辐射扫描电子显微镜和粒子大小分析 (PSA) 分析了沉物.
主要成果:
- 最佳的石沉发生在0.3M尿素和0.3M化下.
- 在这些度下,沉物有97%的石和3%的石.
- 颗粒大小分析显示,大多数颗粒范围在0~60nm之间.
- 较高的反应剂度增加了沉产量,但减少了石的百分比和增加了晶体大小.
结论:
- 不生长的细菌细胞有效地沉了石.
- 反应剂度显著影响石晶体类型,纯度和颗粒大小.
- 优化的生物沉为产业应用提供了一种可控的生产石的方法.
相关概念视频
01:12Precipitation Processes
589
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
589
01:25Crystal Growth: Principles of Crystallization
2.6K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
2.6K
01:10Recrystallization: Solid–Solution Equilibria
1.2K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.2K
01:09Colloidal precipitates
753
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
753
00:52Washing, Drying, and Ignition of Precipitates
1.1K
After filtration, the precipitate is washed to remove coprecipitated impurities and any remaining mother liquor. Colloidal precipitates, such as silver chloride, are washed with an electrolyte (such as dilute nitric acid) to prevent the peptization of the precipitate. In the case of slightly soluble precipitates, the wash solution contains a common ion to reduce solubility. Lead sulfate, which is slightly soluble in water, is washed with dilute sulfuric acid. Similarly, wash solutions may be...
1.1K